Mammalian Expression System

Our company has extensive expertise in providing expression and purification services for recombinant proteins. To provide you with multiple options for protein expression and purification, our researchers have developed specialized expression services for mammalian proteins.

Our company has established a sophisticated mammalian protein expression service platform to provide mammalian eukaryotic protein expression and purification services, including gene expression optimization, transient expression, stable expression and antibody production.

Backgrounds

Mammalian cell system protein expression is a widely used human complex glycosylated protein expression system. Also, it is a great way to express numerous receptor proteins as well as active protein factors. Due to the spatial structure and changes necessary for active proteins, mammalian cell expression systems produce more than 60% of therapeutic protein products.

Mammalian cell expression methods are a superior option in the field of scientific study to produce active recombinant proteins. The mammalian system has many innate advantages and the probability of obtaining naturally modified proteins is greatly increased.

Due to its strong resistance to contamination, high productivity, reliable production cycle, and simple scalability, E. coli is a desirable host system. Our company provides a one-stop shop for high-quality E. coli protein expression and has a wealth of knowledge in the creation and replication of soluble proteins.

Service Overview

Our company is a leading expert in the preparation of various types of proteins and antibodies using our specialty mammalian expression systems. Our one-stop shop provides high-yield, highly expressed proteins with post-translational modifications and proper folding. We offer a range of expression services based on serum-free culture systems, including transient transfection expression, the synthesis of recombinant antibodies, the creation of stable cell lines, the manufacturing of recombinant antibodies, data analysis, and more.

• Transient transfection expression: Multiple mammalian cell expression systems in suspension culture without bleeding greatly increase expression levels (3-6 folds).
• Recombinant antibody expression: We have a proven vector-cell-transfection efficient expression system.
• Large-scale production.

Customers can choose any of the above experiments, or a combination of related experiments, according to their requirements. Choose from affinity purification, ion column purification, hydrophobic packing purification, or molecular sieve purification.

Our specialized high-density cell culture technology, combined with proprietary transfection reagents, media formulations and efficient expression vectors, maximizes cell life, increases expression success and reduces customer costs.

Selection of Mammalian Transient Systems

We specialize in human embryonic kidney 293 (HEK293) and Chinese hamster ovary (CHO) cells. For mammalian transient transfection, HEK293 cells are frequently the ideal option due to their simplicity during transfection, high degree of protein production, and reliable process. CHO cells are excellent for developing therapeutic reagents or for constructing stable production lines in the future. Approximately 70% of all licensed biotherapeutic proteins are produced in stable transfected CHO cells.

If you need a small amount of protein at the milligram level, we recommend transiently transforming HEK293 cells using the pAZ-V5 system; if you need to construct a stable expression cell line, we recommend transiently transforming CHO cells using the pAZ-V5 system and then screening for highly expressed stable cell lines using G418.

If you are looking for smarter, higher quality solutions that incorporate best practices, please feel free to contact us.

It should be noted that our service is only used for research, not for clinical use.