Compared to absorbance and fluorescence, luminescence is a particularly popular detection platform for many applications. Because there is little background interference (autofluorescence from chemicals, medium, and cells), it often has a broader dynamic range and higher sensitivity. Our company offers its customers a professional luminescence testing service.
Our luminescence immunoassay service is a variation of the standard ELISA assay that makes use of low-temperature emitted light (such as chemical or physiological activities). There are many different kinds of luminescence assays, three of which Our company primarily uses are chemiluminescence, electrochemiluminescence, and bioluminescence.
Service Overview
The luminescence immunoassay technique is a method for identifying antigens or antibodies that combines luminescence with immunoreactivity. The method is based on microploidy, and it has good sensitivity and specificity. It offers a wide variety of assays, including those for common proteins, hormones, enzymes, and medications.
In order to assist non-clinical and clinical PK, PD/biomarker, and immunogenicity testing, Our company offers the capacity to conduct a wide variety of luminescence tests for single analytes, multiplex analytes, enzyme activity assays, and cell-based assays. We have the knowledge and skills necessary to create new procedures or to assist in their optimization in order to adhere to legal requirements.
Research Capabilities
Both reactions, chemiluminescence and bioluminescence, are increasingly used to monitor immunoassays. Chemiluminescence is the emission of light during a chemical reaction, and bioluminescence is a specific type of chemiluminescence found in biological systems in which catalytic proteins enhance the efficiency of the chemiluminescence reaction. Light is produced when a molecule formed in the electronically excited state decays to the ground state.
Our chemiluminescence assays and bioluminescence assays both make use of ELISA-like enzymes, such as alkaline phosphatase and horseradish peroxidase (HRP) (AP). The most used substrate for AP is AMPPD (3-(2'-spiroadamantane)-4-methyl-4-(3'-phosphoryloxyphenyl-1,2-dioxetane, disodium salt), while for luminescence tests, substrates such as luminal, polyphenols, and fluorescein are employed for HRP. When electrically triggered in the proper chemical environment, electrochemiluminescent labels like ruthenium emit light.
Overall Solutions
| Project Name | Luminescence Testing Service |
| Test Objectives | - Large Molecules (NBEs)
- Proteins
- Monoclonal Antibodies (mAbs)
- ADCs (Mab, ADA)
- Fusion Proteins
- PEGylated Proteins
- Peptides
- Multi-Valent Antibodies
- Novel Frameworks
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| Deliverables | Original images and raw data.
A complete experimental report, including experimental materials, experimental procedures, and experimental results. |
| Cycle | Decide according to your needs. |
For more information, please feel free to contact us.
It should be noted that our service is only used for research, not for clinical use.
