Humanization is a key step in the development pathway of all therapeutic antibodies derived from non-human sources. Humanized antibodies mostly relate to animal monoclonal antibodies that have undergone re-expression using gene cloning and DNA recombination procedures.
Our company provides professional antibody humanization services by providing a set of humanized variants from your clone that are guaranteed to produce a variant with comparable activity to the parental antibody. Our services are geared at making antibodies easier to manufacture, which increases expression, decreases aggregation, and increases long-term stability and solubility.
Service Overview
Our company uses computer-aided structural simulation to design and humanize your mouse, rabbit, alpaca or alpaca monoclonal antibody into a promising therapeutic lead candidate. We can provide you with the following services, but are not limited to.
- Antibody sequence annotation: Annotation of antibody heavy and light chain variable, constant and CDR regions, identification of antibody germline genes.
- Antibody sequence analysis: Prediction of antibody post-translational modification sites (e.g. glycosylation sites, etc.), prediction of antigenic linear epitopes.
- Antibody structure prediction and optimization: Prediction of structures of full-length antibodies, antibody Fab region and SCFv.
- Humanized antibody design and immunogenicity prediction.
- Antibody-antigen interaction prediction: Identification of conformational epitopes of antigens and determination of hapten attachment sites.
- Design and modification of antibody molecules: Antibody affinity maturation, antibody stability optimization, bispecific antibody design, antibody aggregation effect analysis.
Research Capabilities
Our company's Humanization Approach
Antibodies can be "humanized" by grafting key non-human amino acids onto their antibody framework. This mostly involves the grafting of amino acids into complementarity determining regions (CDRs), but it also involves other amino acids from the framework that are relevant for the VH:VL interface and CDR orientation. Our humanization service strikes a balance between adding as much human components as feasible to lower the danger of immunogenicity and keeping just the right amount of non-human components to preserve the parent antibody's original binding activity.
By integrating humanization with our multi-stage affinity maturation service (STEM TM), our strategy enables us to simultaneously generate superior antibodies. Below is our monoclonal antibody humanization workflow.
(1) Selection of the human germline framework - We integrate the heavy and light chain CDRs of chosen clones into the human framework using bioinformatics analysis. To avoid compromising immunogenicity, we think of amino acids as being tolerated in the human body.
(2) Gene synthesis and recombinant IgG production - We cloned the lead candidate directly into an IgG expression vector and expressed it in HEK293 cells.
(3) Further humanization and affinity maturation using STEM TM - In order to get stronger affinity and specificity, or cross-reactivity to the same antigen in various species for animal investigations, we insert mutations to further humanize the CDR and pan against the target antigen in a progressively strict manner.
Our Advantages
Our company has extensive experience humanizing antibodies and offers professional humanization services with a success guarantee. You won't be charged for our services if we are unable to produce a humanized antibody with activity that is comparable to the parent antibody.
Our services are specialized in offering humanized antibodies that are highly manufactureable, resulting in optimum large-scale production for clinical use. More than 100 humanization projects for clients ranging from modest academic labs to top pharmaceutical companies have been successfully completed by us.
Since then, we have expanded our services to include the humanization of non-animal source species. Our initial strategy concentrated on the humanization of mouse or rat antibodies.
Please feel free to contact us.
It should be noted that our service is only used for research, not for clinical use.
