Amino acids are useful indicators for several disorders as well as for observing the effects of dietary supplements. The amino acid analysis is an appropriate test that not only reliably measures protein levels but also gives in-depth information on the relative amino acid composition and free amino acids if additional applications call for accurate knowledge of protein/peptide amounts.
Our company provides thorough amino acid analysis and quantification services for pharmaceutical samples, biological substances, food, tissue, and biological fluids. The steps we take for amino acid analysis are hydrolysis, isolation, detection, and quantification.
Service Overview
The amino acid analysis is an important tool in all phases of drug discovery and throughout the manufacturing process to ensure consistency between drug batches. The amino acid analysis assays from Our company use derivatization and UPLC-MS/MS to separate and find amino acids in biological matrices like plasma. AccQ-Tag derivatization chemistry enhances sensitivity and chromatographic performance.
We have developed a multiplexed panel of 21 naturally occurring amino acids. This 7-minute approach for amino acid analysis uses UPLC-MS/MS and a straightforward derivatization step to produce excellent sensitivity and peak shape.
Our company has built this method to be used in both non-canonical and canonical formats. We can customize the panel as needed with the possibility of extending it to amino acid content of hydrolyzed proteins or peptides.
Amino acid analysis can be used for:
- Protein concentration calculation and characterization
- Determination of extinction coefficients
- Total amino acids, including cysteine, tryptophan and hydroxyproline
- Free amino acids, including asparagine and glutamine
Research Capabilities
Accurate Protein Quantification
Our service for analyzing amino acids is rather simple: first, a precise sample is hydrolyzed with a powerful acid, and then specific amino acids are identified and measured. All amino acids, with the exception of tryptophan and cysteine, which are broken down during the hydrolysis process, are quantified in this assay. To evaluate your samples, we employ the most recent hardware and software. The outcomes are available in a report that is simple to read.
- For purified proteins with known amino acid sequences
- For complex samples containing unknown proteins
- For protein stock solutions prior to other quantitative analyses
Our amino acid analysis capabilities include:
- Single or triplicate amino acid determinations. We advise running the procedure in triplicate when determining the protein concentration with extreme precision.
- Comparison of results with protein sequences.
Overall Solutions
| Project Name | Amino Acid Analysis Service |
| Service Details | Our services include, but are not limited to, the following: - Quantification of amino acid analysis (quantification of proteins or peptides, quantification of protein traces, accurate quantification of proteins or peptides)
- Determination of amino acid sequences of proteins, peptides and mAbs
- Amino acid composition analysis
- Routine HCL hydrolysis and amino acid analysis
- Buffer blank or abnormal/modified amino acid standard analysis
- Trace analysis of amino acids
- Amino acid analysis data review consulting
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| Deliverables | Original images and raw data.
A complete experimental report, including experimental materials, experimental procedures, and experimental results. |
| Cycle | Decide according to your needs. |
If you want to know more about service prices or technical details, please feel free to contact us.
Related Services
It should be noted that our service is only used for research, not for clinical use.
