Baculovirus Expression Vector System

Our company offers a wealth of expertise in providing services for the expression and purification of recombinant proteins. To give you a variety of options for protein expression and purification, our researchers have developed sophisticated baculovirus-insect cell expression services, including recombinant baculovirus preparation, expression and purification of recombinant proteins and their complexes, as well as a variety of fusion technologies. These services range from protocol design, gene optimization, expression condition optimization, to purification technology systems to improve the expression of proteins.

Backgrounds

The baculovirus expression vector system can deliver high activity and is usually regarded as a potent tool for large-scale protein production due to its stable experimental setup, high expression level, and post-translational modification function. Hundreds of genes, including those from plants and animals as well as viruses, bacteria, and fungi, have so far been effectively expressed in insect cells or larvae.

Fig.1 A baculovirus expression vector system for recombinant protein production. (Mishra V, et al. 2020)

Infection of insect cells with recombinant baculoviruses allows for many eukaryotic post-transcriptional processes of foreign proteins, including glycosylation, phosphorylation, acylation, proper signal peptide cleavage, proteolysis and proper folding. The recombinant protein can properly assemble into oligomers, aggregate, and reside in the same organelle as the natural protein. For the expression of physiologically active proteins, it is therefore the perfect vector.

Service Overview

Our company's baculovirus-insect cell system uses the classic Bac-to-Bac baculovirus expression system to optimize gene sequences and design experimental protocols according to customer needs, and has helped customers with the design of a wide range of cytokines, kinase proteins, multi-domain proteins, and process-modified proteins. Also, our complimentary protein design analysis, gene optimization, and expression protocol suggestions will help you save a ton of time and money.

Our baculovirus vector expression system services include, but are not limited to, the following. Customers can choose any of the above experiments, or a combination of related experiments, according to their requirements.

• Preparation of recombinant baculovirus DNA (3 weeks).
• Preparation of recombinant baculovirus (2 weeks).
• Determination of recombinant virus titers (2-3 days).
• Optimization of recombinant protein expression conditions (2 weeks).
• Expression and purification of small-scale proteins (2-4 weeks).
• Expression and purification of large-scale proteins (2-4 weeks).
• Insect cell culture in bioreactors (Determine based on the expressed yield and volume).

Why Choose Us

With our optimized baculovirus-insect cell expression system, our team has extensive experience in customizing a variety of challenging protein targets, including but not limited to virulence proteins, kinases, intracellular proteins, and membrane proteins.

The advantages of our baculovirus-insect system include:

• Cost-effective.
• Easily scalable.
• Can be subjected to a variety of post-translational modifications.
• Suitable for large protein targets.
• Suitable for challenging targets, such as kinases and toxic proteins.

If you are looking for smarter, higher quality solutions that incorporate best practices, please feel free to contact us.

Reference

1. Mishra V, et al. (2020). "A Comprehensive Guide to the Commercial Baculovirus Expression Vector Systems for Recombinant Protein Production." Protein & Peptide Letters. 27(6): 529-537.

It should be noted that our service is only used for research, not for clinical use.