Enzymology and Molecular Interaction Research Service

Enzymes power practically all cellular functions, including DNA replication, gene transcription, and metabolism, to name a few. Enzymology, the study of enzymes and enzyme-catalyzed reactions, is a multidisciplinary science.

With this in mind, Our company offers specialized enzymatic and analytical interaction research services to support biological research in molecular biology, kinetic analysis, structural biology, and computational biology.

Service Overview

Our company has a wealth of experience in the field of in vitro biology and offers a full range of biology services, such as measurements of enzymes at the cellular level, cell biology, biochemistry, and in vitro isotopes, as well as the establishment of stable cell lines and the use of gene knockdown, RNAi, and microRNA technologies.

Our studies of enzymes and their functions include:

• Molecular structure (primary, secondary, tertiary, and quaternary)
• Protein properties (isoelectric point, electrophoretic mobility, pH, temperature, stability, and spectral properties)
• Kinetics (specificity, velocity, and reversibility)
• Thermodynamic properties (free energy and entropy of activation)
• Active sites (the number of available sites, the molecular nature of the sites, and the mechanism of catalysis)
• Biological properties (location/compartment in the cell, form of isoenzyme, and metabolic impact of the reaction performed)

Our in vitro enzymology and molecular interaction technology platform includes:

• luminescence analysis methods (Kinase-Glo, ADP-Glo, etc.)
• Absorption analysis methods (UV and VI)
• Non-labeling selection methods (SPR)

Below is a list of some of the enzymatic targets that have been validated by Our company.

Molecular Interaction Platform

Our company uses the Biacore 8K high-throughput test system to help customers analyze large volumes of samples in a short period of time. The system offers high throughput and high sensitivity. We can help our customers perform surface plasmon resonance (SPR) testing of biological samples to help them understand molecular function and screen for effective molecules.

Applications

• Micromolecular drug screening
• Functional assessment of organisms
• Large molecule force detection
• Assay Indicators
• Binding specificity analysis
• Kinetic analysis
• Concentration analysis
• Affinity analysis

If you are looking for smarter, higher quality solutions that incorporate best practices, please feel free to contact us.

It should be noted that our service is only used for research, not for clinical use.