Protein Chip Detection Service

Protein microarray (protein chip) technology has become a powerful new tool for protein research that can quickly generate a significant volume of repeatable and trustworthy data. The captured protein array is often attached to a support surface, such as a slide, nitrocellulose membrane, beads, or microtitre plate, in microarrays.

Our company provides customized protein chip detection services to support a range of applications in fundamental and clinical research, including disease marker investigations, protein modification and enzyme substrate profiling, protein interaction profiling, as well as DNA/RNA binding protein screening.

Service Overview

One of the main advantages of protein microarrays over traditional tools such as ELISA and mass spectrometry is that they are highly sensitive to a wide range of target concentrations and require lower sample volumes than antibody microarrays. Our company currently offers two types of protein microarrays: analytical and functional protein microarrays.

• Analytical protein microarrays, primarily antibody microarrays, have become one of the most powerful multiplex assay technologies.
• Functional protein microarrays are increasingly being used in many areas of biodiscovery, including the study of protein interactions, biochemical activity, and immune responses.

Our protein chip detection service is implemented using high-density commercial chips and offers the following comparative advantages.

• Chip standard: 1×25×76 mm silicon base, protein spot diameter ~60 μm, volume 0.5~1 nL.
• Protein chip: high signal-to-noise ratio, good stability, maintain natural protein conformation as much as possible, high specificity.
• Technical reproducibility: 2 technical reproducibilities per protein spot.
• Versatility: We use fluorescent reporter groups of different wavelengths to detect multiple reactants for each immobilized protein on the chip.
• Purification system: All proteins are expressed and purified by eukaryotic yeast expression system under non-denaturing conditions.
• Production conditions: temperature 4~8 ℃, humidity 30~40%, overnight fixation at 4 ℃, long-term storage at -80 ℃.

Research Capabilities

Protein Interaction Profile Screening

Our high-throughput proteomics microarrays are a powerful tool for protein interaction profile identification. The interaction of decoy proteins with ~20000 proteins coated with clear background information on the microarray can greatly increase the scope and efficiency of screening for interacting proteins.

DNA/RNA Binding Protein Screening

Our microarray technology can rapidly locate proteins bound to target nucleic acids and the rich biological information behind them, playing an important role in studies of transcription factor screening, RNA toxicity, and pathogenic microbial invasion.

Disease Marker Research: Autoantibody Profiling and Screening

Proteomic microarrays cover about 20000 human proteins that can be combined with and screened against these human autoantibodies on a large scale, thus providing a wealth of candidate markers for diagnosis, prognosis and drug efficacy evaluation of various diseases.

Protein Modification and Enzyme Substrate Profiling Identification

The proteomics microarray contains ~20000 full-length human proteins expressed by eukaryotic yeast. These proteins are biologically active enough to modify a wide range of proteins. The enzymatic reactions provide a large number of candidate substrates, each with a clear background of biological information about the protein.

Small Molecule and Monoclonal Antibody Drug Target Screening

Our protein microarray technology can rapidly analyze qualitatively and relatively quantitatively the binding of small molecule or monoclonal antibody drugs labeled with fluorescent, biotin, or radioisotope to ~20,000 human full-length proteins to determine the binding targets and specificity of candidate molecules, providing a powerful technology for the improvement of the drug development system.

An antibody chip is a type of protein microarray. For more information about our services, please click Antibody Chip Detection.

If you are looking for smarter, higher quality solutions that incorporate best practices, please feel free to contact us.

Reference

1. Khandelwal R, et al. (2018). "Structure-based Virtual Screening for the Identification of High-affinity Small Molecule Towards STAT3 for the Clinical Treatment of Osteosarcoma." Current Topics in Medicinal Chemistry. 18(29): 2511-2526.

It should be noted that our service is only used for research, not for clinical use.