Our company has extensive expertise in providing expression and purification services for recombinant proteins. In order to provide you with multiple options for protein expression and purification, our researchers have developed specialized expression services for Escherichia coli (E. coli) systems.
Our company has established a proven E. coli expression service platform to provide efficient and cost-effective production of various recombinant proteins and their complexes for expression and purification in E. coli.
Backgrounds
Expression of proteins in E. coli is the most common method for producing recombinant proteins. Because of its high output, low cost, and simplicity of usage, E. coli is a common host choice. The E. coli protein expression system is an important instrument for the growth of the biotechnology sector and recombinant protein expression, particularly for the generation of tiny proteins. It is applicable to a wide range of species.
Fig.1 Schematic representation of the E. coli expression system for producing highly O-GlcNAcylated proteins. (Gao H, et al. 2018)
Due to its strong resistance to contamination, high productivity, reliable production cycle, and simple scalability, E. coli is a desirable host system. Our company provides a one-stop shop for high-quality E. coli protein expression and has a wealth of knowledge in the creation and replication of soluble proteins.
Service Overview
Our company has extensive experience in E. coli systemic protein expression and offers E. coli recombinant protein expression that is productive and cost-effective. Given the fact that some foreign genes form inclusion bodies of membrane-encapsulated, high-density insoluble proteins after recombinant expression in E. coli, we will optimize our expression systems to minimize the probability of inclusion body formation.
Our E. coli protein expression system services include, but are not limited to, the following. Customers can choose any of the above experiments, or a combination of related experiments, according to their requirements. Choose from affinity purification, ion column purification, hydrophobic packing purification, or molecular sieve purification.
- Construction of a recombinant plasmid
- Recombinant expressor tests
- Soluble expression test
- Expression and purification of small-scale proteins
- Expression and purification of large-scale proteins
We will perform mild inclusion body replication by evaluating and determining the replication conditions. We provide the purity of the complexed proteins above 95%. We can provide expression of characterized fusion proteins with SUMO tags. For customers who do not require a tag, we can cut the tag without leaving a residue. Our unique low temperature induced expression system increases the probability of expression of soluble proteins, improving the probability of expression success and activity of protein products.
Principles of E. coli Protein Expression
Expression vector
Small circular DNA can self-replicate. A complete plasmid vector must have a replication source, a promoter, an inserted target gene, a selection marker and a terminator. Depending on the type of expressed protein, they can be divided into simple expression vectors and fusion expression vectors.
Expression of host bacteria
The selection of host bacteria is mainly based on the characteristics of the host bacteria and the characteristics of the target protein. For example, the target protein needs to form disulfide bonds. Customers can choose the Origami 2 series, which can greatly increase the possibility of disulfide bond formation in the cytoplasm and improve the solubility and activity of the protein.
If you are looking for smarter, higher quality solutions that incorporate best practices, please feel free to contact us.
Reference
- Gao H, et al. (2017). "A Widely Compatible Expression System for The Production of Highly O-GlcNAcylated Recombinant Protein in Escherichia Coli." Glycobiology.28(12): 949-957.
It should be noted that our service is only used for research, not for clinical use.
