Short-chain Fatty Acid Targeted Metabolomics Analysis

Short-chain fatty acid (SCFA), a kind of saturated fatty acid with five or fewer carbon atoms, are often made by anaerobic bacteria through the process of glycolysis. SCFA has important functions such as regulation of blood glucose and control of drug release by hybridization with monosaccharide molecules. Diabetes, cancer, and neurological disorders can all be brought on by abnormalities in SCFA metabolism.

Our company provides targeted metabolomic analysis of SCFA based on gas chromatography-mass spectrometry (GC-MS) technology to perform absolute qualitative and quantitative analysis of 7 SCFAs in stool/content/blood samples to reveal drug mechanism of action and assist in the diagnosis and treatment of clinical diseases.

Service Overview

Based on the Agilent GC-MS 7890B-5977A analytical platform, Our company has successfully developed a reliable analytical method that, in combination with various fatty acid isotope standards, enables quantitative and qualitative analysis of a wide range of fatty acids. We can provide analytical services for 7 SCFAs including acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, and capric acid.

Our company has participated in dozens of targeted metabolomics analysis projects for SCFAs and has accumulated extensive experience in assays and analyses. Our targeted metabolomics analysis services not only significantly improve the accuracy of qualitative and quantitative analysis, but also greatly increase the throughput and stability.

Our company will eventually provide a detailed technical report that includes:

• Experimental procedures
• Relevant mass spectrometry parameters
• Quantitative or quantitative information of the identified substance
• Mass spectral maps
• Raw data

Sample Requirements

• Animal and clinical tissue specimens: 200 mg/sample, liquid nitrogen flash frozen at -80 ℃, avoid repeated freezing and thawing, dry ice delivery.
• Serum and plasma: 200 μL/sample, snap frozen in liquid nitrogen and stored at -80 ℃, sent on dry ice.
• Urine: 1 mL/sample, snap frozen in liquid nitrogen and stored at -80 ℃. Send on dry ice.
• Microorganisms, cells: 1×10⁷ cells/sample, snap frozen in liquid nitrogen and stored at -80 ℃, sent on dry ice.
• Feces: 200 mg/sample liquid nitrogen snap frozen and stored at -80 ℃, sent on dry ice.
• Recommended biological replication
  (1) Clinical samples: ≥ 30 cases per group
  (2) Animal source: ≥ 10 cases per group
  (3) Plant origin: ≥ 3 cases per group
  (4) Cellular samples: ≥ 6 cases per group
• Please consult technical support for other sample types.

Our Features

• Advanced platform: High sensitivity, high qualitative capability, stability and reliability, helping to achieve precise target identification.
• Proprietary kit: Specially developed for short-chain fatty acids to achieve absolute quantification.
• Strict quality control: Standard QC and Sample QC systems to ensure data stability/accuracy.
• Professional data pre-processing: Differential compound screening and PCA pattern identification
• Wide linearity range: 0.1-200 ug/mL with a correlation coefficient >0.99, which can meet different types of complex sample quantitative analysis.
• Manual calibration: Strict manual proofreading process to ensure accuracy and reliability of the method.
• Professional service: Pre-sales and post-sales professional and reliable guarantee, data publication responsible to the end.

Fig.1 Determination of short-chain fatty acids and branched-chain amino acids in stool and urine samples from newborn and lactating mothers by targeted GC-MS method. (Ramos-Garcia V, et al. 2022)

Application Areas

• Disease research: research into intestinal inflammation, colon cancer and other diseases.
• Study of intestinal flora-related mechanisms: mechanisms of metabolic regulation by SCFAs.

If you are looking for smarter, higher quality solutions that incorporate best practices, please feel free to contact us.

Reference

1. Ramos-Garcia V, et al. (2022). "GC-MS Analysis of Short Chain Fatty Acids and Branched Chain Amino Acids in Urine and Faeces Samples from Newborns and Lactating Mothers." Clinica Chimica Acta. 532: 172-180.

It should be noted that our service is only used for research, not for clinical use.