Microbial Targeted Metabolomics Analysis

Microbial metabolomics is the study of the whole set of metabolites within a microbe. Each bacterium typically has a proteome and genome that are quite tiny. However, their metabolomes are all incredibly distinctive and more accurately represent the functions of the cells. As a result, taxonomic and functional studies of microorganisms frequently use microbial metabolomics.

Our company is pleased to offer reliable services for microbial-focused metabolomics analysis. We determine the best sample preparation strategy based on your requirements, ensuring effective metabolite extraction and extremely precise microbial metabolomics analysis.

Service Overview

Our company's microbial targeted metabolomics analysis focuses on the identification and quantification of targeted small molecules, including microbial primary metabolites and secondary metabolites. Our service plan is a complete solution, from sample pre-treatment to data analysis.

We will first perform preliminary experiments on the client's samples. In the initial experiments, we will use a variety of different metabolic molecule extraction methods and perform mass spectrometry analysis of the samples obtained from each extraction method. The raw data obtained will be subjected to an in-depth bioinformatics analysis. The obtained metabolic molecules are matched with the metabolic molecules of your interest.

Based on the matching results, we select one or two suitable extraction methods for subsequent formal batch sample processing and analysis. The customer only needs to provide enough samples to obtain scientific results in a timely manner. We can also customize the solution according to the customer's requirements.

Our company will eventually provide a detailed technical report that includes:

• Experimental procedures
• Relevant mass spectral parameters
• Mass spectra graphs
• Raw data
• Microbial metabolomics results

Research Capabilities

Our company's microbial metabolomics focuses on the analysis of intracellular metabolites. Due to the active intracellular enzyme system and rapid metabolite conversion, the method of sampling and sample preparation will greatly affect the accuracy and reproducibility of analytical results. We select appropriate quenching methods based on the characteristics of different microorganisms, some examples of which are listed below.

Our company's Technology Platforms

• Nuclear magnetic resonance (NMR)
• Gas chromatography/mass spectrometry (GC/MS)
• Liquid chromatography/mass spectrometry (LC/MS)
• High performance liquid chromatography/mass spectrometry (HPLC/MS)
• Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF)

Sample Requirements

For the overall analysis of metabolites, sample extraction should meet the following requirements.

• Extraction of as many metabolites as possible.
• Be free of bias.
• No destruction or alteration of the physical or chemical properties of the metabolite.

The workflow of Our company's sample pretreatment method for obtaining cellular metabolites includes:

(1) Inhibition of metabolism by quenching.
(2) Sample collection, separation of extra- and intracellular metabolites
(3) Metabolite extraction

Fig.1 General metabolite sample preparation methods workflow for laboratory scale bacterial metabolomics study. (Kamal K. M, et al. 2022)

If you are looking for smarter, higher quality solutions that incorporate best practices, please feel free to contact us.

Reference

1. Kamal K. M, et al. (2022). "Bacterial Metabolomics: Sample Preparation Methods." Biochem Res Int. 2022: 9186536.

It should be noted that our service is only used for research, not for clinical use.