Short-term Repeated Dose Dermal Toxicity Test

Short-term repeated dermal toxicity is defined as the health detrimental effect caused by repeated percutaneous exposure for a period shorter than 10% of the life span of the experimental animal.

Our company offers a professional short-term repeated dose dermal toxicity (28-day) testing service that provides information on the health hazards caused by repeated percutaneous exposure to test samples over a defined test period.

This service provides customers with a preliminary understanding of the percutaneous permeability, toxicity characteristics, and target organs of the samples, as well as the no-observed-adverse-effect level (NOAEL) and lowest-observed-adverse-effect level (LOAEL) in terms of dose-effect and dose-response relationships, providing a basis for the selection of doses and observations for subchronic and chronic percutaneous toxicity tests.

Test Range

Veterinary drugs, pesticides, chemicals, fungicides, organic toxicants, solid waste, domestic waste leachate, hazardous waste, sewage, sludge, water quality, surfactants, etc.

About Short-term Repeated Dose Dermal Toxicity Service

Test Methods

The dorsal area of the animal is debrided prior to staining, and as often as necessary throughout the staining period. We apply different doses of the test sample to the skin of the animals daily. The animals are contaminated for 28 days.

We observe the toxicity response of the animals daily, weigh and calculate the feeding amount regularly, and performed hematological, biochemical and histopathological measurements to evaluate the short-term repeated percutaneous toxicity of the samples and to initially determine the dose and target organs of the test substance causing harmful effects in the animals.

Subjects

• For liquid samples, we generally use the original liquid.
• For solid samples, we crush them into powder form and moisten them well with water or other media that are non-toxic, non-irritating, do not affect skin permeability, and do not react with the sample to ensure good contact with the skin.

The samples are diluted and prepared before they are about to be stained.

Test Animals

We usually use adult rats, rabbits or guinea pigs for the tests. The weight range is 200-300 g for rats, 2.0-3.0 kg for rabbits, and 350-450 g for guinea pigs, with at least 10 animals of each sex with healthy skin in each dose group. Females are chosen to be non-pregnant and unborn.

Test Observations

• Clinical observations: Changes in skin and coat, eye and mucous membrane changes, respiratory nutrient system, circulatory system, vegetative and central nervous system, limb movements and behavioral patterns.
• Body weight and food intake were recorded.
• Hematologic examination: Hemoglobin concentration, red blood cell count, hematocrit, total white blood cell count and classification, platelet count, and coagulation function. Reticulocytes and bone marrow smear cytology should be added if there is an effect on the hematological system.
• Blood biochemical examination: Electrolyte balance, carbohydrate metabolism, liver function, special function, etc. The test index should include at least alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine (Cr), blood glucose (GLU), albumin (ALB), total protein (TP), total cholesterol (TCH), potassium, and sodium.
• Urine examination: Appearance, specific gravity, acidity and alkalinity, urine protein, glucose and blood cells, etc.
• Pathological examination: Gross anatomical examination and histopathological examination.
• Other tests: If necessary, other sensitive indicators are added according to the nature of the test sample and the observed toxic reaction.

Short-term Repeated Dose Dermal Toxicity Test Report

The final test report Our company provides includes the following.

1. Test name, test start and end date, test project leader, etc.
2. Test summary.
3. Test sample name, physical and chemical properties, solvent or medium used for preparation, preparation method and other specific information.
4. Specific information on the experimental animals and experimental animal facilities.
5. Dose and group: The principle or basis for choosing the dose, dose and group, the way animals are grouped and the number of animals of each sex in each group.
6. Infection method: Infection dose, infiltration area, infiltration density, infiltration method and microclimate of the infiltration environment, such as room temperature, humidity, wind speed, etc.
7. Test results: Clinical manifestations of poisoning by sex and dose, death, observed appearance and duration of each abnormal manifestation and its regression, results of hematological and blood biochemical examinations, detailed description of gross anatomical and histopathological examinations, statistical methods for processing the test results.
8. Test conclusions: Conclusions on toxic effects and toxic effects target organs of the tested samples, as well as NOAEL and LOAEL.

If you are looking for the best solution in the field of toxicology research, please feel free to contact us.

It should be noted that our service is only used for research, not for clinical use.