Acute Dermal Toxicity Test

Our company provides acute dermal toxicity testing services to test the acute toxicity of the samples on experimental animals and to provide a basis for subacute (chronic) toxicity and micronucleus testing.

Test Purpose

We provide this service to determine whether the test samples are absorbed through the skin and the toxicological response produced in the short term, which can provide the basis for toxicity classification and labeling of the ingredients, as well as identifying sub-chronic toxicity tests and other toxicological test formulations.

Test Range

Veterinary drugs, pesticides, chemicals, fungicides, organic toxicants, solid waste, domestic waste leachate, hazardous waste, sewage, sludge, water quality, surfactants, etc.

Test Items

Test Methods

Our company offers the following acute dermal toxicity test items:

• Acute dermal toxicity test for pesticides.
• Acute dermal toxicity test for cosmetics.
• Acute dermal toxicity test for chemicals.
• Acute dermal toxicity of chemicals fixed dose test.
• Medical device systemic toxicity test (acute dermal toxicity test).

About Acute Dermal Toxicity Test Service

Test Method

Prior to the acute dermal toxicity test, Our company removed the coat of the test animals at the test site. The test animals are divided into at least three groups and each group is coated with a different dose of the test substance, and then observed for toxic reactions and mortality. The animals that died during the test and those that were executed at the end of the test should be subjected to gross deprogramming and the median lethal dose (LD₅₀) and 95% confidence interval should be calculated.

We will exclude the systemic effects caused by local skin irritation or corrosive effects caused by the samples in the test.

Subjects

Liquid samples generally do not have to dilute and can be directly tested with the original solution.

Solid samples are insoluble or insoluble solids or granular and should be ground and sieved through 100 mesh. Water or other non-toxic, non-irritating, does not affect the permeability of the skin, but also does not react with the sample medium fully wetted, to ensure that the sample has good contact with the skin. Commonly used excipients are water, vegetable oil, petroleum jelly, lanolin, etc.

Test Animals

Rats are preferred, but guinea pigs or rabbits can be used. The body weights of the animals were 200-300 g for rats, 350-450 g for guinea pigs, and 2000-3000 g for rabbits.

Observation Index

We will observe once a day for 14 consecutive days, not exceeding 21 days.

Observations will include the central nervous system and neuromuscular system, vegetative nervous system, respiratory system, genitourinary system, skin and coat, digestive system and eyes, as well as body weight weighing (before, after and before execution) and toxicological examination.

Evaluation of Test Results

• The samples can be absorbed percutaneously and make a preliminary evaluation of the toxic effect characteristics.
• Evaluate the level of toxic effects of the samples, if the dose-response relationship curve can be obtained, a preliminary evaluation of the threshold of acute dermal toxic effects can be made.
• To clarify the toxicity parameters obtained with what sex of animals and exposure time.

Acute Dermal Toxicity Test Report

The final test report we provide includes the following.

1. Name of the sample, the active ingredient, purity (or content), dosage form, date of manufacture, physical and chemical properties, solvent used for preparation and method.
2. Species, strain, grade, number, weight, sex, and source of experimental animals.
3. Laboratory animal feeding environment, including temperature, relative humidity, feed, single cage feeding or group feeding, and laboratory animal facilities license number.
4. Dose and group, including the principle or basis for selecting the dose, dose and group, the way the animals are grouped and the number of animals of each sex in each group.
5. Test conditions and methods, including the main instruments and equipment, the route of poisoning, poisoning protocol, test period, observation index, etc.
6. Test results, including clinical manifestations of poisoning, time of appearance, duration and recovery of poisoning manifestations, time of death after poisoning, weight change, correlation with dose and gross anatomical and histopathological changes, etc., and the statistical treatment of the results.
7. Conclusion of the test, including clear conclusions such as LD₅₀, 95% confidence interval and toxicity classification.

If you are looking for the best solution in the field of toxicology research, please feel free to contact us.

It should be noted that our service is only used for research, not for clinical use.