Mammalian Spermatogonial and Spermatocyte Chromosome Aberration Test

Our company offers a mammalian spermatogonial/spermatocyte chromosome aberration test service, which uses cytogenetic methods to detect the effect of chromosomal aberrations in mammalian germ cells caused by customer samples in order to evaluate their potential to cause genetic mutations in germ cells.

Test Range

Veterinary drugs, pesticides, chemicals, fungicides, organic toxicants, solid waste, domestic waste leachate, hazardous waste, sewage, sludge, water quality, surfactants, etc.

About Mammalian Spermatogonial/Spermatocyte Chromosome Aberration Test Service

Test Method

The animals are exposed to the samples by appropriate routes and after a certain time, the animals were executed. The animals are treated with a mid-phase cytokinesis blocker before execution, and after execution, both testes are removed. The spermatogonia/primary spermatocyte chromosome specimens are prepared after hypotonicity, fixation, softening and staining.

The mid-phase cytokinesis phase cells are observed under a microscope and the spermatogonia/primary spermatocyte chromosome aberrations are analyzed to evaluate the mutagenicity of the test samples on male germ cells.

Sample Preparation

Solid samples are dissolved or suspended in an appropriate solvent or carrier and diluted. Liquid samples can be used directly or after dilution. Samples are freshly prepared.

We determine the solvents used for the samples based on the physicochemical properties of the samples, using dose levels that are not toxic to experimental animals and do not react chemically with the samples. Usually, we use distilled water, isotonic saline, vegetable oil, edible starch, sodium carboxymethyl cellulose, etc.

Test Animals

Healthy young sexually mature male mice and Chinese hamsters are routinely used for our germ cell chromosome aberration tests. Other suitable male mammals can also be used upon request.

Control Group

The negative control group is treated the same as the sample group except that no test sample is used.

Positive control animals should develop detectable above-background chromosomal structural aberrations in spermatogonia/primary spermatocytes in vivo. We use positive control compounds that correlate with the chemical classification of the samples. Commonly used positive controls are cyclophosphamide, cyclophosphamide monohydrate, mitomycin C, triethylenemelamine, and multiple doses of the positive control group.

Mammalian Spermatogonial/Spermatocyte Chromosome Aberration Test Report

The final test report Our company provides includes the following.

1. Test summary.
2. Information on sample name, purity, physicochemical properties, preparation method, etc.
3. Specific information such as experimental animals and feeding environment.
4. Test conditions and methods: Basic principles of dose grouping and dose selection, information on positive and negative controls, route and mode of poisoning, sampling time points, name of blocking agent and concentration used and treatment time, operation steps, statistical methods used, etc.
5. Test results: Symptoms of intoxication, types of chromosomal aberrations and number of aberrant cells per animal, the total number of cellular aberrations per group, mean and standard deviation, number of each type of aberration per cell per group, dose-response relationship, etc.
6. Conclusion: Based on the test results, conclusions are made on whether the samples had mutagenic effects under the test conditions.

For more information, please feel free to contact us.

It should be noted that our service is only used for research, not for clinical use.