In vivo Mammalian Bone Marrow Cell Chromosome Aberration Test

Chromosomes are small bodies in the nucleus that have a specific structure and genetic function. When chemicals are applied to the G1 and S phases of the cell cycle, they induce chromosomal aberrations, and when they are applied to the G2 phase, they induce chromosomal aberrations.

Our company offers a professional in vivo mammalian bone marrow cell chromosome aberration test service. This is a mutagenicity test that can be used to evaluate the mutagenic potential of a customer's sample for mammalian bone marrow cell chromosome aberrations.

This service is particularly applicable to chromosomal aberration analysis that requires consideration of metabolic activation in vivo. This service is not applicable if there is evidence that the test or its metabolites do not reach the bone marrow.

Test Range

Veterinary drugs, pesticides, chemicals, fungicides, organic toxicants, solid waste, domestic waste leachate, hazardous waste, sewage, sludge, water quality, surfactants, etc.

About Mammalian Bone Marrow Cell Chromosome Aberration Test Service

Test Method

We expose the experimental animals to the samples through appropriate pathways and execute the animals after a certain period of time. Before execution, colchicine is injected into the peritoneal cavity to inhibit the formation of spindle filaments during cytokinesis in order to increase the proportion of cells in the mid-phase division and to shorten, disperse, and outline the chromosomal filaments.

After execution, we remove bone marrow and prepare chromosome specimens, which are later observed under a microscope to analyze chromosomal aberrations in mid-phase division cells.

Sample Preparation

We prefer distilled water as the solvent. If the samples are insoluble in water, edible oil, edible starch, and 0.5% sodium carboxymethyl cellulose are used to prepare emulsions or suspensions.

The test samples are usually freshly configured before the test.

Test Animals

We recommend the use of healthy primary adult rats, mice, and Chinese hamsters, other suitable mammals can also be used.

Infection Method

We often use gavage. Positive controls can also be injected intraperitoneally. We can use other methods of infection depending on the client's needs.

Control Group Setup

• We set up positive and negative controls for each test and treat them the same as the sample group except that no test sample is used.
• Common positive controls include ethyl methanesulfonate, ethyl nitrosourea, mitomycin C, cyclophosphamide, etc.
• The negative control group consists of solvent or carrier.

Mammalian Bone Marrow Cell Chromosome Aberration Test Report

The final test report Our company provides includes the following.

1. Test name, test start and end date and other information.
2. Summary of the test.
3. Information such as the name of the test sample, active ingredient, physical and chemical properties, and preparation method;
4. Specific information such as experimental animals and feeding environment.
5. Dose and group: The principle or basis for selecting the dose, the dose and group, the way of grouping animals and the number of animals of each sex in each group.
6. Test conditions and methods: Main instruments and equipment, route of poisoning, methods of poisoning, specimen preparation, etc.
7. Test results: The poisoning performance of each group, mitotic index, chromosome aberration type and the number of each group, its mean and standard deviation, calculation method and statistical analysis of data of each group, dose-response relationship, etc.
8. Conclusion of the test: The conclusion of whether the test has a mutagenic effect under the test conditions is given.
9. Description of the original record keeping.

For more information, please feel free to contact us.

It should be noted that our service is only used for research, not for clinical use.